Category: BMR NGS

NGS

About us

University of Padua, spin off

BMR Genomics, spin-off of the University of Padua, is a DNA Sequencing provider operating in Italy since 2006, and offering Next Generation Sequencing services since 2008.

The quality procedures adopted to handle samples are certified ISO 9001; we have a custom Laboratory Information Management System (LIMS), developed in house, and a highly automated sample management using Beckman pipetting robots.

Call us!

To contact us you can write an e-mail to nextgen@bmr-genomics.it or call us at:
+39 049 0995752

A partner for projects

BMR Genomics has been a partner for several EU projects (Aquatrace, Fishboost, MD Paedigree, Metable…). We are a SME and an experienced sequencing provider, with a proven ability manage the EU commission requirement to keep tasks and work packages on time.

quality cerfitication iso 9000

The quality certification of the company is a crucial step to avoid sample mishandling.

Company Data

BMR Genomics srl – Via Redipuglia 21A, 35131 Padova, Italia
C.S. 80000 € i.v. – REA 345372

P.IVA/VAT: 03888370289


NGS

Requirements for DNA extraction service

We offer a service of DNA extraction starting from complex matrices using MoBio PowerFecal® extraction kit. This kit can be used interchangeably with the PowerSoil® DNA Isolation kit to isolate DNA from soil, environmental, stool, gut and biosolid samples. For large sample size we use automated Qiacube HT robot with MoBio sample preprocessing (lysis step).

Matrices requirements:

  • Stools: 100 – 200 mg in 2 ml tubes (Vials);
  • Biosolids and soils: 100 – 200 mg in 2 ml tubes (Vials);
  • Whey, milk and beverages: 2 ml in 2 ml tubes (Vials);
  • Fresh dairy products: 250 – 500 mg in 2 ml tubes (Vials);
  • Oral microbiome: 25 – 50 mg of lyophilized saliva/oral rinse in 2 ml tubes (Vials).

SA11_72694005_300x300

 Example of required Vial, screw cap.

 

If your matrix is not present in this list, we need to test our protocols on few of your samples (one or two) and provide the first amplification step using different DNA diluitions. This set up has a fixed price of 250 euros.

Shipment

Matrices to be extracted (but dry powders) must be sent us in refrigerated boxes (from 4°C to -20°C).

For more information contact us at nextgen@bmr-genomics.it.


NGS

Universal 16S Primer pairs

We provide three different standard primer pairs:

V3-V4 (for Bacteria – Nadkarni et al.,2002)
16S_univFor: 5′-CCTACGGGAGGCAGCAGT-3′
16S_univRev: 5′-GACTACCAGGGTATCTAATCCTGTT -3′

V3-V4 (for Bacteria and Archaea – Takahashi et al. 2014)
Pro341F: 5′-CCTACGGGNBGCASCAG -3′
Pro805R: Rev 5′-GACTACNVGGGTATCTAATCC -3′

V1-V3 (for Bacteria – McMurtry et al.,2015)
27 F: 5′-GAGTTTGATCNTGGCTCAG -3′
519R: 5′-GWNTTACNGCGGCKGCTG -3′