BMR Genomics

Welcome to our NGS website

NGS

Microbial genome sequencing

Sequencing a novel genome enables molecular studies, addressing exciting questions spanning from adaptation to pathogenesis.

Our de novo genome sequencing service is complete: we prepare the libraries, perform the sequencing on the robust Illumina platform, and we perform the assembly and preliminary annotation.

What we do

  • Whole genome shotgun library
  • Illumina MiSeq sequencing, 300 bp paired-end
  • Sequencing QC
  • De novo genome assembly (producing contigs)
  • Automatic gene prediction and annotation using PROKKA 1.11
  • Personal BLAST server [optional]

Requirements

  • 1.0 ug genomic DNA
  • 260/280 1.8-2.0
  • Intact DNA, agarose gel checked

Should you work with difficult samples, giving lower yields of total RNA, please contact us to arrange custom specifications.

What we deliver

You’ll receive:

  • The raw sequences, in FASTQ format
  • The contigs produced by the de novo assembly steps, in FASTA format
  • The tabular coordinates of predicted genes, from gene prediction
  • The genome annotation in both GFF and GBK (GeneBank) format

What we need to know

Before writing us for a quote, To prepare our best service offer, we need to know:

  1. Your experimental goal (what are you looking for). The “standard” approach gives good results for re-sequencing of known bacteria and to produce a draft for novel genomes. If you are looking for genes or genome rearrangements we can propose scaffolding and finishing steps.
  2. If there are closely related genomes sequenced, and/or if you are resequencing a strain of well known bacteria
  3. The estimated genome size (in Mb)
  4. The number of organisms you wish to sequence.

Prices

Contact us for more info at nextgen@bmr-genomics.it,  at +39 049 0995752 or using the following form: